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Differential scanning calorimetry of 3-fold mutants. DSC scans and corresponding endotherms of WT P-II (1), E344Q P-II (2), E348A P-II (3), E344A/E348A (double mutant) (4), and E344A (5). The scans are overlaid with corresponding melting temperatures shown above (Tm). The Tm for E344A is shown as not determined (ND) because of its unusual endotherm.
