Fig. 1.

Mass spectrum of intact ribosomes from T. thermophilus. The mass spectrum of intact ribosomes from T. Thermophilus in 1 m ammonium acetate showing well resolved charge states for the 30 S subunit at 14,000–18,000 m/z and 70 S subunit at 25,000–28,000 m/z is shown. The 50 S subunit lacks charge state resolution probably because of the heterogeneity associated with the partial loss of the stalk complex, which appears as a series of well resolved charge states at 4000–6000 m/z. Individual ribosomal proteins and tRNA are observed at the lower m/z regions of the spectrum.