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. 2010 May 13;9(8):1774–1783. doi: 10.1074/mcp.M000072-MCP201

Fig. 7.

Fig. 7.

Mass spectra of ribosomal stalk complexes with either heptameric or pentameric stoichiometry and with both present simultaneously. M. vannielii is one of the species for which we observed both pentameric and heptameric ribosomal stalk complexes simultaneously (bottom panel). For other species strictly one stoichiometry was observed as illustrated by stalk complexes from B. stearothermophilus (pentamer; middle panel) and T. thermophilus (heptamer; top panel). All complexes were buffer-exchanged into 1 m ammonium acetate solution at pH 7.5 before introducing into the mass spectrometer.