Table I. Measured and calculated masses for proteins from stalk complexes of various species together with stoichiometry defined by MS/MS.
Where masses of the component proteins were not known a priori, the molecular mass of the stalk complex was calculated from the measured masses of the constituent proteins.
| Species, particle studied, optimal growth temperature | Mass of the stalk complex (Da) | Masses of protein released in MS/MS (Da) | Predicted mass (Da) | Identity | Stoichiometry |
|---|---|---|---|---|---|
| Bacteria | |||||
| B. subtilis, 70 S, 38.8 °C | 68,501.3 ± 18.0 | 68,374 | Pentamer | 4:1 | |
| 12,623.1 ± 0.15 | 12,619 | L12-Met | |||
| 17,952.79 ± 0.4 | 18,029 | L10-Met | |||
| 55,836.7 ± 7.2 | 55,755 | Pentamer-L12 | |||
| 43,204.0 ± 3.4 | 43,136 | Pentamer-2 L12 | |||
| T. aquaticus, 50 S, 70–75 °C | 97,658.4 ± 24.2 | 95,883 | Heptamer | 6:1 | |
| 12,937.1 ± 0.3 | 12,908a | L12 | |||
| 18,435 ± 0.5 | 18,509a | L10 | |||
| B. stearothermophilus, 70 S/50 S, 55 °C | 69,481.25 ± 26.4 | 4:1 | |||
| 12,835.60 ± 1 | 12,780 | L12 | |||
| 17,863.79 ± 0.2 | L10 | ||||
| 56,441.96 ± 1.4 | Pentamer-L12 | ||||
| 51,511.66 ± 2 | Pentamer-L10 | ||||
| Eukaryotes | |||||
| A. salina brine shrimp, 60 S, 28 °C | 80,479.25 ± 9.3 | Pentamer | 4:1 | ||
| 34,309.48 ± 7.9 | P0 | ||||
| 11,440.38 ± 1.39 | 11,407 | P1-Met | |||
| 11,522.16 ± 0.7 | 11,503 | P2 | |||
| 68,737.2 ± 13.3 | Stalk-P1/P2 | ||||
| 57,390.5 ± 22.9 | Stalk-2(P1/P2) | ||||
| 46,073.1 ± 16.3 | Stalk-P0 | ||||
| O. cuniculus rabbit, 60 S, 39 °C | 81,115.89 ± 48.3 | Pentamer | 4:1 | ||
| 34,298.25 ± 1.95 | 34,216b | P0 | |||
| 11,810.15 ± 7.98 | 11,651b | P1 | |||
| 11,433.35 ± 0.48 | 11,475b | P2 | |||
| 11,510.63 ± 3.72 | 11,525b | P2c | |||
| 69,135 ± 60.0 | Pentamer-P1 | ||||
| 69,604.3 ± 32.7 | Pentamer-P2 | ||||
| Galdieria sulphuraria red alga, 60 S, 56 °C | 86,244 ± 32.1 | 4:1 | |||
| 82,880 ± 14.43 | 81,675 | Pentamer | |||
| 34,312.9 ± 12.7 | 34,763c | P0 | |||
| 11,778.4 ± 5.8 | 11,615c | P1 | |||
| 11,902.6 ± 1.7 | 11,835c | P2 | |||
| 70,283.7 ± 6.7 | Pentamer-P1/P2 | ||||
| 58,0431.5 ± 61.0 | Pentamer-2 P | ||||
| Archaea | |||||
| M. vannielii, 50 S, 35 °C | 75,342.7 ± 20.7 | 75,223 | Pentamer | 4:1 | |
| 9,819.71 ± 2.4 | 9,818 | L12 | |||
| 65,588.71 ± 31 | 65,405 | Pentamer-L12 | |||
| 55,729.02 ± 9.9 | 55,587 | Pentamer-2 L12 | |||
| 95,405.27 ± 23.6 | 94,859 | Heptamer | 6:1 | ||
| 9,818.93 ± 0.04 | 9,818 | L12 | |||
| 85,359.78 ± 25.53 | 85,041 | Heptamer-L12 | |||
| 75,445.09 ± 38.5 | 75,223 | Heptamer-2 L12 | |||
| M. barkeri, 70 S, 37 °C | 80,124.39 ± 18.0 | 80,040 | Pentamer | 4:1 | |
| 10,677.21 ± 0.41 | 10,677 | L12 | |||
| 69,455.71 ± 2.63 | 69,363 | Pentamer-L12 | |||
| 101,650.02 ± 30.7 | 101,394 | Heptamer | 6:1 | ||
| 90,762.69 ± 18.55 | 90,717 | Heptamer-L12 | |||
| M. maripaludis, 70 S, 35–40 °C | 74,814.80 ± 4.99 | 74,785 | Pentamer | 4:1 | |
| 9,745.39 ± 1.5 | 9,745 | L12 | |||
| 65,090.93 ± 6.77 | 65,040 | Pentamer-L12 | |||
| 55,333.38 ± 4.04 | 55,295 | Pentamer-2 L12 | |||
| 95,159.52 ± 15.63 | 94,275 | Heptamer | 6:1 | ||
| 84,603.44 ± 9.93 | 84,530 | Heptamer-L12 |
a From Thermus thermophilus, a related species.
b From Mus musculus (mouse), a related species.
c From Cyanidioschyzon merolae, a related species.