(A) Predicted miR-200b binding sites in the 3′UTRs of Suz12 and Bmi1 with sequence complementarity and phylogenic conservation of 8-nt seed sequence indicated. Zeb1 and Zeb2 show similar complementarity as Suz12 with the exception of Drosophila.
(B) MiR-200b, Suz12 and Bmi1 RNA levels (mean ± SD) in untransformed, transformed non-stem cancer cells (NSCCs), CSCs, and mammospheres (MSP).
(C) RNA levels (mean ± SD) of the indicated genes after transfection with miR-200b or control microRNAs in CSCs.
(D) Suz12 and β-actin protein levels after transfection with miR-200b or control microRNAs in CSCs.
(E) Relative luciferase activity (mean ± SD) mediated by reporter constructs harboring the 3′UTR of the indicated genes (and mutated version of Suz12) upon transfection with miR-200b or control microRNAs.