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. 2010 Jul;9(7):1616–1632. doi: 10.1074/mcp.M000153-MCP201

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 10. — Phosphorylation statuses of TRAP1 and RIP in NPM-ALK knockdown ALK⁺ ALCL cells. A, NPM-ALK siRNA was used to decrease the expression level of NPM-ALK in SU-DH-L1 cells. The scrambled siRNA-transfected SU-DH-L1 was used as a negative control. The expression of NPM-ALK was detected by using anti-ALK antibody. The expression levels of TRAP1 and RIP were detected using anti-TRAP antibody and anti-RIP antibody, respectively. B, immunoprecipitation (IP) experiments were performed using NPM-ALK knockdown SU-DH-L1 with anti-TRAP1 antibody and anti-RIP antibody, respectively. The phosphorylation statuses of TRAP1 and RIP were detected using anti-phosphotyrosine antibody. The scrambled siRNA-transfected SU-DH-L1 cell lysates were used as controls. IB, immunoblot; phos, phospho.