Fig. 7.

RT-PCR analyses of selected proteins of unknown function found to be induced under anaerobic conditions from the SILAC quantitative data show varying inductions at transcript level, whereas immunoblot detection of CF1 β subunit of chloroplast ATP synthase, LHCSR3, TEF7, and PsaD shows consistencies with SILAC data. A, Cgld22, cpld51*, and tef5 are GreenCut proteins (21). Cgld22 (JGI v3.1: 102133) is a protein similar to ATP synthase I, cpld51* (JGI v3.1: 120574) is a protein required for cytochrome b₆f assembly, and tef5 (JGI v3.1: 192099) has a Rieske [2Fe-2S] domain. Tef7 is a Chlamydomonas-specific protein of unknown function. Protein 114525 is a prolyl 4-hydroxylase*, protein 183968 has a weak homology to PsbP, and protein 190196* is annotated as a signal peptide peptidase-like protein. TEF5 and protein 114525* have been annotated as having oxidoreductase activity. B, immunoblot analysis of 5 μg of chlorophyll of AR, AN, 4-day sulfur-deprived anaerobic cultures (4d AN −S), and 6-day sulfur-deprived anaerobic cultures (6d AN −S) using αCF1, αLHCSR3, αTEF7, and αPsaD antibodies. The immunoblot demonstrates induction of TEF7 under all anaerobic conditions, whereas LHCSR3 is induced only under sulfur-deprived anaerobic conditions.