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. 2001 Feb 13;98(4):2059–2064. doi: 10.1073/pnas.98.4.2059

Figure 1.

Figure 1

RFLP patterns detected by the Pto gene in wild L. hirsutum, L. esculentum, and near-isogenic L. esculentum lines. Genomic DNA (3 μg) isolated from each of the indicated lines was digested with EcoRI, separated on a 1% agarose gel, and transferred onto Hybond N+ membrane. The membrane was hybridized to a 32P-labeled PCR product of the Pto open reading frame and washed to a stringency of 0.5× SSC at 65°C. Rio Grande-PtoR and TA537 contain the Pto locus from L. pimpinellifolium, Rio Grande-PtoS and TA209 contain the Pto locus from L. esculentum, and 96T133-3 contains the Pto locus from the L. hirsutum tomato accession PI134418.