Table 2.

Agrobacterium-mediated transient expression assays of the LhirPto genes

Gene	HR
LhirPto	−
avrPto	−
Pto/avrPto	+
LhirPto/avrPto	+
Lhirfen/avrPto	−
LhirPtoD/avrPto	−
LhirPtoF/avrPto	−

The indicated genes were placed under control of the cauliflower mosaic virus 35S promoter and cloned into the pBTEX plasmid. These constructs were transformed into Agrobacterium tumefaciens strain EHA105. A. tumefaciens strains were induced with acetosyringone and syringe-infiltrated into mature Nicotiana benthamiana leaves. Leaves were scored for HR 4 days after infiltration. + indicates that total tissue collapse occurred in the infiltrated tissue; − indicates that no tissue collapse was observed.