Fig. 1.

Association of virus filaments and inclusion bodies in virus-infected cells. Virus-infected cells were labeled using antibodies to P protein (green) and F protein (red). A series of images was obtained from the same cell at different focal planes in the Z-axis by confocal microscopy. The Z-stacks were then processed and visualized in three-dimensional as described under “Experimental Procedures.” A is a single optical slice visualized in parallel to the substrate and viewed two-dimensionally. B is a low magnification deconvolved image showing a region near the surface of the cell, again parallel to the substrate. The inclusion body (IB) can be seen to be irregular in shape and labeled only by anti-P, whereas the virus filaments (VF) are labeled by both anti-P and anti-F. C is a higher magnification image within the cell showing an individual inclusion body and its associated virus filaments. The three-dimensional projection has now been rotated through 90° and tilted slightly, showing that the filaments are predominantly above the inclusion body and in a vertical orientation. * highlights the smaller sized inclusion body.