Table I. Kinases for which motifs are significantly enriched in this phosphopeptide data set.
Statistical information for the identified motifs is provided in supplemental Table S3. The highly enriching motifs were queried against the Human Protein Reference Database using the PhosphoMotif Finder (34). Putative yeast homologs of the human kinases were identified through the HomoloGene Database (35) and searches of the Saccharomyces Genome Database (36).
| Feature of motif described in literature | Instances | S. cerevisiae homolog |
|---|---|---|
| Casein kinase II substrate motif | 155 | Yck1p, Yck2p, Yck3p, Cka1p, Cka2p, Ckb1p, Ckb2p |
| Casein kinase I substrate motif | 77 | Hrr25p |
| G-protein-coupled receptor kinase 1 substrate motif | 57 | Gpr1p, Gpa2p, Gpb1p, Gpb2p |
| MAPKAPK2 kinase substrate motif | 52 | MAPK |
| PKA kinase substrate motif | 48 | Tpk1p, Tpk2p, Tpk3p, Bcy1p |
| GSK3 kinase substrate motif | 26 | Mck1p, Rim11p, Mrk1p, Ygk3p |
| PKC kinase substrate motif | 25 | Pkc1p |
| GSK-3, ERK1, ERK2, CDK5 substrate motif | 22 | Mck1p, Rim11p, Mrk1p, Ygk3p, Fus3p, Kss1p, Pho85p |
| Calmodulin-dependent protein kinase II substrate motif | 8 | Cmk1p, Cmk2p |
| Unknown | 3 |