Table IV. Details of RLC N-terminal peptide 9–30 mouse and human PTMs measured via LC-MS/MS. Modified amino acid residues are shown in bold.
p, phosphorylation; d, deamidation; Spec Mill, Spectrum Mill; mods, modifications.
| Species | Peptide sequence | Precursor m/z | Precursor MH+ | Precursor charge | Δppm | SPI | Spec Mill score | No. detected mods |
|---|---|---|---|---|---|---|---|---|
| % | ||||||||
| Mouse | RIEGGSSNVFSMFEQTQIQEFK | 854.75 | 2562.2396 | 3 | 5.8 | 96 | 20.8 | 0 |
| Mouse | RIEGG(sS)NVFSMFEQTQIQEFK | 881.40 | 2642.2115 | 3 | 4.2 | 91 | 20.8 | 1 p |
| Mouse | RIEGGssNVFSMFEQTQIQEFK | 908.06 | 2722.1702 | 3 | 3.0 | 96 | 19.2 | 2 p |
| Human | RAGGANSNVFSMFEQTQIQEFK | 830.40 | 2489.1875 | 3 | −0.1 | 100 | 21.0 | 0 |
| Human | RAGGAnSNVFSMFEQTQIQEFK | 830.73 | 2490.1817 | 3 | 4.0 | 93 | 20.2 | 1 d |
| Human | RAGGANsNVFSMFEQTQIQEFK | 857.05 | 2569.1498 | 3 | −1.7 | 81 | 18.4 | 1 p |
| Human | RAGGAnsNVFSMFEQTQIQEFK | 857.38 | 2570.1356 | 3 | −1.0 | 89 | 19.1 | 1 d, 1 p |