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. 2010 May 28;9(9):1968–1981. doi: 10.1074/mcp.M900430-MCP200

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 5. — Relative proportion of glycan residues on apoE(192–206) and apoE(283–299) peptides of cellular and secreted apoE glycoforms separated by 2-DE. Immunoprecipitated cellular apoE and secreted apoE were separated by 2-DE, apoE glycoform spots were excised after Coomassie staining and destained, and XICs were obtained. Proportional glycan content on cellular (a) and secreted (b) apoE(192–206) peptide and cellular (c) and secreted (d) apoE(283–299) peptide was determined for each glycoform and is presented as a percentage of total residues detected within each 2-DE glycoform spot.