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. 2010 May 28;9(9):1968–1981. doi: 10.1074/mcp.M900430-MCP200

Table II. LTQ-FT MS analysis of glycosylated apoE peptides from apoE glycoforms isolated by 2-DE.

2-DE assignment Glycan Peptide AATVGSLAGQPLQER (mass, 1496.79)
 Peptide VQAAVGTSAAPVPSDNH (mass, 1619.8)
Retention time Mass (0 charge) Theoretic mass Mass difference Retention time Mass (0 charge) Theoretic mass Mass difference
ppm ppm
1 HexNAc 34.38 1699.88 1699.87 1.818 30.63 1822.87 1822.87 1.053
2 Hex-HexNAc-NeuAc 27.51 2276.02 2276.02 −0.909
Hex2-(HexNAc)2-NeuAc 31.07 2518.16 2518.17 −3.157
3 Hex-HexNAc-NeuAc 27.53 2276.02 2276.02 −1.424
Hex-HexNAc-(NeuAc)2 31.09 2444.12 2444.12 −1.342
4 Hex-HexNAc-(NeuAc)2 29.36 2567.12 2567.121 −1.223
Hex2-(HexNAc)2-(NeuAc)2 33.4 2809.25 2809.26 −2.100
5 Hex-HexNAc-(NeuAc)2 26.99 2567.12 2567.12 −0.721
Hex2-(HexNAc)2-(NeuAc)2 30.87 2809.24 2809.26 −5.614
6 Hex-HexNAc-(NeuAc)2 29.35 2567.12 2567.12 −1.024
Hex2-(HexNAc)2-(NeuAc)2 33.42 2809.25 2809.26 −3.083
7 Hex-HexNAc-(NeuAc)2 29.36 2567.11 2567.12 −2.555
Hex2-(HexNAc)2-(NeuAc)2 33.41 2809.25 2809.26 −3.189
8 None 35.29 1496.80 1496.80 1.243 31.32 1619.79 1619.79 0.407
9 Hex-HexNAc-NeuAc 29.99 2276.02 2276.02 0.211
Hex2-(HexNAc)2-NeuAc 33.6 2518.16 2518.17 −2.144
10 Hex-HexNAc-(NeuAc)2 29.36 2567.11 2567.12 −2.006
Hex2-(HexNAc)2-(NeuAc)2 33.41 2809.25 2809.26 −3.798
11 Hex-HexNAc-(NeuAc)2 33.79 2444.12 2444.12 −1.428 29.49 2567.12 2567.12 −0.802
12 Hex-HexNAc-(NeuAc)2 29.42 2567.12 2567.12 0.016
Hex2-(HexNAc)2-(NeuAc)2 33.37 2809.26 2809.26 −1.951
13 Hex-HexNAc-(NeuAc)2 33.72 2444.12 2444.12 −1.943 29.37 2567.12 2567.12 −0.464
14 Hex-HexNAc-(NeuAc)2 33.68 2444.13 2444.12 1.052 29.39 2567.11 2567.12 −1.971