Table II. Identification of deregulated proteins in tumorous cholangiocytes.
| Accession number | Protein name | Gene name | -Fold change cancer/normal | Sequence coverage | Unique peptides/proteotypic peptides | Protein score | Postulated biological processa |
|---|---|---|---|---|---|---|---|
| % | |||||||
| Proteins having peptides detected with increased abundance in tumorous samples | |||||||
| VIME_HUMAN | Vimentin | VIM | 9.26 | 68.5 | 39/34 | 2,496 | Cell motion |
| LDHB_HUMAN | l-Lactate dehydrogenase B chain | LDHB | 7.72 | 37.4 | 11/10 | 808 | Monocarboxylic acid metabolic process |
| Q5JP53_HUMAN | Tubulin β polypeptide | TUBB | 4.76 | 33.8 | 16/3 | 1,098 | Cell motion |
| Q1WM23_HUMAN | Nucleoside-diphosphate kinase | NME1 | 3.66 | 32.6 | 8/8 | 431 | Regulation of cell proliferation, metastatic process |
| LDHA_HUMAN | l-Lactate dehydrogenase A chain | LDHA | 3.45 | 34.0 | 10/9 | 620 | Anaerobic glycolysis |
| S10AB_HUMAN | Protein S100-A11 (calgizzarin) | S100A11 | 3.01 | 43.8 | 5/5 | 293 | Signal transduction, regulation of cell proliferation |
| ALDOA_HUMAN | Fructose-bisphosphate aldolase A | ALDOA | 2.28 | 31.0 | 10/10 | 531 | Actin cytoskeleton organization, hexose metabolic process |
| KPYM_HUMAN | Pyruvate kinase M2 | PKM2 | 2.17 | 45.2 | 20/20 | 1,410 | Hexose metabolic process |
| A8K220_HUMAN | Peptidyl-prolyl cis-trans isomerase (cyclophilin) | PPIA | 2.16 | 50.3 | 11/11 | 811 | Protein folding |
| K1C18_HUMAN | Keratin, type I cytoskeletal 18 | KRT18 | 2.12 | 64.9 | 33/32 | 2,571 | Cell morphogenesis |
| PROF1_HUMAN | Profilin-1 | PFN1 | 1.97 | 55.0 | 8/8 | 457 | Actin cytoskeleton organization |
| Q6FGI1_HUMAN | Transgelin-2 | TAGLN2 | 1.86 | 33.2 | 6/6 | 355 | Actin cytoskeleton regulation |
| PTMA_HUMAN | Prothymosin α | PTMA | 1.86 | 21.6 | 2/2 | 126 | Transcription |
| COF1_HUMAN | Cofilin-1 | CFL1 | 1.70 | 50.0 | 6/6 | 433 | Actin cytoskeleton organization |
| MDHC_HUMAN | Malate dehydrogenase, cytoplasmic | MDH1 | 1.68 | 29.9 | 8/8 | 517 | Carboxylic acid metabolic process |
| HS90B_HUMAN | Heat shock protein HSP 90-β | HSP90AB 1 | 1.47 | 15.5 | 11/5 | 739 | Molecular chaperone, protein folding |
| Proteins having peptides detected with decreased abundance in tumorous samples | |||||||
| AGR2_HUMAN | Anterior gradient protein 2 | AGR2 | 0.09 | 22.3 | 4/4 | 208 | Unknown |
| CAH2_HUMAN | Carbonic anhydrase II | CA2 | 0.09 | 25.4 | 6/6 | 353 | Response to osmotic stress |
| ANX13_HUMAN | Annexin A13 | ANXA13 | 0.23 | 33.9 | 9/9 | 480 | Cell differentiation |
| A8K008_HUMAN | cDNA FLJ78387 | 0.26 | 11.2 | 4/4 | 237 | Unknown | |
| A0A5C9_HUMAN | IGLV2-14 protein | IGLV2-14 | 0.31 | 12.4 | 2/2 | 107 | Unknown |
| K1C19_HUMAN | Keratin, type I cytoskeletal 19 | KRT19 | 0.32 | 59.3 | 32/21 | 2,330 | Actin cytoskeleton organization |
| EST1_HUMAN | Liver carboxylesterase 1 | CES1 | 0.32 | 34.0 | 16/16 | 1,091 | Metabolic process |
| LEG4_HUMAN | Galectin-4 | LGALS4 | 0.35 | 12.7 | 3/3 | 187 | Cell adhesion |
| HBA_HUMAN | Hemoglobin subunit α | HBA1 | 0.39 | 39.4 | 6/6 | 328 | Oxygen transport |
| Q06AH7_HUMAN | Transferrin | TF | 0.45 | 5.7 | 4/4 | 172 | Iron ion transport |
| AT1A1_HUMAN | Sodium/potassium-transporting ATPase subunit α1 | ATP1A1 | 0.49 | 25.1 | 19/19 | 1,244 | Cellular ion homeostasis, carcinoma cell motility |
| ANXA4_HUMAN | Annexin A4 | ANXA4 | 0.49 | 56.4 | 23/23 | 1,885 | Signal transduction, antiapoptosis |
| ERLN2_HUMAN | Erlin-2 | ERLIN2 | 0.50 | 10.6 | 3/3 | 180 | Unknown |
| ALBU_HUMAN | Serum albumin | ALB | 0.51 | 56.5 | 39/39 | 2,418 | Transport |
| A4GX73_HUMAN | Hemoglobin subunit β | HBB | 0.51 | 75.5 | 9/4 | 678 | Oxygen transport |
| A4D1PA_HUMAN | Aldo-keto reductase family 1, member B10 | AKR1B10 | 0.55 | 13.6 | 4/4 | 271 | Cellular lipid metabolic process |
| TALDO_HUMAN | Transaldolase | TALDO1 | 0.56 | 14.8 | 5/5 | 213 | Carbohydrate metabolic process |
| A0A5E5_HUMAN | IGKC protein | IGKC | 0.57 | 24.2 | 4/4 | 304 | Immune response |
| AK1C1_HUMAN | Aldo-keto reductase family 1 member C1 | AKR1C1 | 0.62 | 14.6 | 3/3 | 116 | Steroid metabolic process |
| HSP71_HUMAN | Heat shock 70-kDa protein 1 | HSPA1A | 0.63 | 27.5 | 15/4 | 975 | Ubiquitin proteasome pathway, protein folding |
| PDIA3_HUMAN | Protein-disulfide isomerase A3 | PDIA3 | 0.68 | 26.3 | 11/11 | 646 | Protein folding |
| ANXA2_HUMAN | Annexin A2 | ANXA2 | 0.79 | 53.1 | 17/17 | 1,189 | Actin cytoskeleton organization |
| A1A5C4_HUMAN | RRBP1 protein | RRBP1 | 0.80 | 10.6 | 9/9 | 427 | Translation |
a Postulated biological process was determined by gene ontology and bibliographic features.