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. 2010 Apr 24;9(9):1898–1919. doi: 10.1074/mcp.M900494-MCP200

Table I. S. aureus COL secreted proteins identified in present study.

Numbers in the superscript indicate the different forms of the protein identified. Theor., theoretical; Sig P, signal peptide, seq., sequence.

Gene ID Protein name Theor. mass Predicted massa Observed massb Mass error Modifications Trap location MS/MS seq. cov.c
Da Da Da Da %
Cytotoxins
    SACOL1173d α-Hemolysin precursor (HlY) 35,973.3 33,260.1 33,260.6 0.5 −Sig P Trap 6 84
    SACOL2003d β-Hemolysin (Hlb) 37,237.8 33,742.6 33,742.8 0.1 −Sig P Trap 7 90
    SACOL20221e δ-Hemolysin (Hld1) 2,976.6f 2,976.6f 2,976.6f 0.0 100
    SACOL20222e δ-Hemolysin (Hld2) 2,976.6f 3,020.6f 3,020.6f 0.0 +N-terminal formylation, +16 Da 100
    SACOL2419g γ-Hemolysin, component A (HlgA) 34,955.7 31,921.9 Trap 10 53
    SACOL24221d γ-Hemolysin, component B (HlgB1) 36,711.0 33,392.0 33,392.6 0.6 −Sig P, truncated protein Trap 4 69
    SACOL24222 γ-Hemolysin, component B (HlgB2) 36,711.0 33,462.1 33,463.6 1.5 −Sig P, truncated protein Trap 4 69
    SACOL24223 γ-Hemolysin, component B (HlgB3) 36,711.0 33,506.2 33,507.4 1.2 −Sig P, truncated protein Trap 4 69
    SACOL2421d γ-Hemolysin, component C (HlgC) 35,625.8 32,565.2 32,566.2 1.0 −Sig P Trap 9 85
    SACOL1880d Leukotoxin LukD (LukD) 36,888.9 34,158.6 34,159.1 0.5 −Sig P Trap 6 53
    SACOL1881d Leukotoxin LukE (LukE) 34,819.1 31,750.4 31,751.5 1.1 −Sig P, −C-terminal residue Asn Trap 8 68
    SACOL2004d Leukocidin subunit precursor, putative 38,686.1 35,573.3 35,574.4 1.1 −Sig P Trap 8 74
    SACOL20061d,h Aerolysin/leukocidin family protein 40,434.0 37,619.7 37,620.8 1.1 −Sig P Trap 10 72
    SACOL20062h Aerolysin/leukocidin family protein 40,434.0 37,418.4 37,419.3 0.9 −Sig P Trap 10 73
Superantigenic toxins
    SACOL0442 Staphylococcal enterotoxin 23,165.4 19,343.9 19,345.0 1.1 −Sig P Trap 8 69
    SACOL0886d Staphylococcal enterotoxin (Sek) 27,727.1 24,698.4 24,699.6 1.2 −Sig P, −C-terminal residues YKETI Trap 12 82
    SACOL0887d Staphylococcal enterotoxin type I (Sei) 28,184.6 24,846.6 24,848.0 1.4 −Sig P, −C-terminal residues TE Trap 20 77
    SACOL0907d Staphylococcal enterotoxin B (Seb) 31,435.8 28,368.0 28,368.5 0.5 −Sig P Trap 8 100
Proteases
    SACOL1869d Serine protease SplA (SplA) 25,876.2 21,853.5 21,854.4 0.9 −Sig P Trap 7 70
    SACOL1868d Serine protease SplB (SplB) 26,096.4 22,371.1 22,372.0 0.9 −Sig P Trap 6 66
    SACOL1867d Serine protease SplC (SplC) 26,098.4 22,388.0 22,388.7 0.7 −Sig P Trap 4 48
    SACOL1866d Serine protease SplD (SplD) 25,669.1 22,010.8 22,012.2 1.4 −Sig P, seq. error Trap 5 77
    SACOL1865d Serine protease SplE (SplE) 25,679.3 22,011.9 22,013.3 1.4 −Sig P Trap 7 32
    SACOL1864d Serine protease SplF (SplF) 25,655.1 21,941.7 21,942.7 1.0 −Sig P Trap 5 93
    SACOL1057d,g V8 protease (SspA) 36,312.6 33,376.1 Trap 5 42
    SACOL1056d Cysteine protease precursor SspB (SspB1) 44,519.0 40,649.4 40,650.5 1.1 −Sig P Trap 5 78
    SACOL1970d,g Cysteine protease precursor SspB (SspB2) 44,252.1 41,524.8 Trap 12 56
    SACOL2659d,g Zinc metalloproteinase aureolysin (Aur) 56,361.3 53,459.8 Trap 8 48
Lipolytic enzymes
    SACOL2694d Lipase1 (Lip1) 76,675.3 73,077.3 73,078.0 0.8 −Sig P Trap 8 96
    SACOL0317d Lipase2 (Lip2) 71,276.8 67,152.0 67,152.8 0.8 −Sig P Trap 9 71
    SACOL0078d 1-Phosphatidylinositol phosphodiesterase (Plc) 37,086.7 34,127.1 34,128.0 0.9 −Sig P Trap 6 90
Peptidoglycan hydrolases
    SACOL0263d Peptidoglycan hydrolase (LytM) 35,067.4 31,735.5 31,736.5 1.0 −Sig P Trap 4 66
    SACOL0507d,g N-Acetylmuramoyl-l-alanine amidase (Sle1) 35,835.7 33,424.9 Trap 12 76
    SACOL07231d LysM domain protein 28,186.8 11,848.9 11,849.0 0.1 −Sig P, N-terminal fragment Trap 2 100
    SACOL07232 LysM domain protein 28,186.8 13,800.9 13,801.8 0.9 −Sig P, C-terminal fragment Trap 2 72
    SACOL10621d Bifunctional autolysin (Atl1) 137,334.9 134,248.2 134,249.5 1.3 −Sig P Trap 9 99
    SACOL10622 Bifunctional autolysin (Atl2) 137,334.9 80,786.9 80,787.3 0.4 −Sig P, proteolytic processing Trap 8 99
    SACOL10623 Bifunctional autolysin (Atl3) 137,334.9 53,479.5 53,479.7 0.2 −Sig P, proteolytic processing Trap 9 99
    SACOL2088d SceD protein (SceD) 24,096.0 21,497.0 21,497.7 0.7 −Sig P Trap 3 76
    SACOL2584d Immunodominant antigen A (IsaA) 24,203.2 21,377.9 21,377.5 −0.4 −Sig P Trap 3 91
    SACOL2666d N-Acetylmuramoyl-l-alanine amidase domain protein 69,253.2 42,250.8 42,252.1 1.3 −Sig P, protein degradation Trap 6 89
Da Da Da Da %
Miscellaneous enzymes
    SACOL0303d Acid phosphatase 5-nucleotidase 33,351.9 30,184.3 30,185.0 0.7 −Sig P Trap 11 87
    SACOL0860 Thermonuclease precursor (Nuc) 25,119.9 18,782.3 18,782.0 −0.3 −Sig P Trap 7 97
    SACOL0962d Glycerophosphoryl diester phosphodiesterase (GlpQ) 35,310.7 32,240.2 32,241.0 0.8 −Sig P Trap 10 100
    SACOL10711h Chitinase-related protein 11,344.8 8,720.8 8,721.0 0.2 −Sig P Trap 3 91
    SACOL10712h Chitinase-related protein 11,344.8 8,906.9 8,907.1 0.2 −Sig P Trap 3 89
    SACOL10713h Chitinase-related protein 11,344.8 9,902.0 9,901.6 −0.4 −Sig P Trap 3 92
Surface adhesins
    SACOL08581 Secretory extracellular matrix and plasma-binding protein (Empbp1) 38,484.9 12,890.5 12,891.8 1.3 −Sig P, N-terminal fragment Trap15 73
    SACOL08582 Secretory extracellular matrix and plasma-binding protein (Empbp2) 38,484.9 22,709.8 22,708.9 −1.0 −Sig P, C-terminal fragment Trap 15 53
    SACOL0985d Surface protein, putative 15,838.2 12,859.7 12,859.5 −0.3 −Sig P Trap 4 100
    SACOL1164 Fibrinogen binding-related protein 12,596.6 9,592.1 9,592.5 0.4 −Sig P Trap 3 69
    SACOL1168 Fibrinogen-binding protein (Efb) 18,764.6 15,850.1 15,851.0 0.9 −Sig P Trap 10 90
    SACOL2002 Map protein (Map) 76,945.2 73,877.7 Trap 17 31
    SACOL2019 SdrH protein, putative (SdrH) 46,630.0 38,083.8 38,085.1 1.2 −Sig P, removal of C-terminal residues 377–419 Trap 3 85
    SACOL2197d Surface protein, putative 15,447.5 12,480.0 12,480.1 0.1 −Sig P Trap 3 100
    SACOL2418d IgG-binding protein (Sbi) 50,070.2 47,049.7 47,049.3 −0.4 −Sig P Trap 9 95
    SACOL2660 Immunodominant antigen B (IsaB) 19,370.2 15,785.0 15,785.3 0.3 −Sig P Trap 3 100
Unknown functions
    SACOL0270d Staphyloxanthin biosynthesis protein, putative 33,032.2 30,379.1 30,377.9 −1.2 −Sig P, −42-Da modification Trap 12 59
    SACOL0271 Virulence factor EsxA (EsxA) 11,306.2 10,905.2 10,905.2 0.2 −Met Trap 3 100
    SACOL0480 Hypothetical protein 11,301.8 8,380.3 8,380.4 0.1 −Sig P Trap 9 100
    SACOL0669d Conserved hypothetical protein 18,594.2 15,905.0 15,905.4 0.4 −Sig P Trap 6 100
    SACOL0755d Conserved hypothetical protein 16,922.1 13,449.9 13,449.1 −0.8 −Sig P Trap 7 79
    SACOL0859 Hypothetical protein 17,717.0 14,812.5 14,813.4 0.9 −Sig P Trap 7 67
    SACOL0908d Hypothetical protein 20,345.8 16,271.1 16,271.1 0.0 −Sig P Trap 5 99
    SACOL1166d Hypothetical protein 15,202.5 12,304.0 12,304.0 0.0 −Sig P Trap 5 55
    SACOL2179 Conserved hypothetical protein 32,763.6 29,134.0 29,135.5 1.5 −Sig P Trap 8 42
    SACOL22911d Staphylococcal secretory antigen SsaA2 29,327.1 12,211.3 12,210.3 −1.0 −Sig P, N-terminal fragment Trap 3 98
    SACOL22912 Staphylococcal secretory antigen SsaA2 29,327.1 14,294.5 14,294.9 0.4 −Sig P, C-terminal fragment Trap 3 97
    SACOL2295d Staphyloxanthin biosynthesis protein 17,424.8 14,755.7 14,756.7 1.0 −Sig P, +16-Da modification Trap 3 71
    SACOL2557 Conserved domain protein 16,870.3 12,788.5 12,789.5 1.0 −Sig P Trap 6 61

a Predicted masses were calculated by subtracting the mass of the signal peptide from the theoretical mass. For proteins with additional modifications, recalculated predicted mass is presented in the table.

b Average mass of the protein from replicate analyses.

c Net sequence coverage of the mature protein.

d Protein identified in other S. aureus strains (29).

e Protein identified in the extracellular peptide extract by peptide MS/MS analysis; the reported protein mass was calculated from precursor ion mass.

f Monoisotopic mass.

g Protein could not be identified by whole protein MS analysis.

h Protein with signal peptide cleavages at more than one site.