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. 2010 Jun 1;9(9):1819–1828. doi: 10.1074/mcp.2010/000893

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© 2010 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 5. — Immunoblot analysis of TIMP1 levels in lung cancer patients' sera before or after ConA lectin-column chromatography. (A) Serum levels of TIMP1 were analyzed by immunoblot analysis using anti-TIMP1 antibody. 20 μg each of the immunodepleted serum samples were prepared from six each of healthy volunteers (Normal), COPD patients, stage-I/II lung cancer patients, or stage-IV lung cancer patients and used for the blotting. Samples used in the IGEL-iTRAQ screening experiments were indicated by the iTRAQ reporter numbers (113 to 121). (B) Immunoblot of TIMP1 in the ConA-bound fractions prepared from the same sample set in (A). The entire ConA-bound fraction from 75 μg of total protein was loaded for each lane. Samples used in the IGEL-iTRAQ screening experiments were indicated by the iTRAQ reporter numbers (113 to 121).