Table I. List of 8 glycopeptides showing more than 2-fold affinity change to ConA lectin in lung cancer patients' sera.
| Accessions | m/z (z) | aConf. | bSequence | Modifications | c113/Navr | c114/Navr | c115/Navr | c116/Navr | c117/Navr | c118/Navr | c119/Navr | c121/Navr |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P02750|A2GL_HUMAN | 722.12 (+3) | 99 | K143LPPGLLAN151*FTLLR156 | iTRAQ8plex@N-term | 0.487 | 0.977 | 1.771 | 0.430 | 1.818 | 4.298 | 8.601 | 3.339 |
| iTRAQ8plex(K)@1 | ||||||||||||
| User modH on Asn(N)@9 | ||||||||||||
| P02750|A2GL_HUMAN | 653.32 (+2) | 98 | M286FSQN290*DTR293 | iTRAQ8plex@N-term | 0.588 | 0.996 | 1.686 | 0.530 | 2.433 | 6.324 | 6.919 | 2.442 |
| User modH on Asn(N)@5 | ||||||||||||
| P08571|CD14_HUMAN | 599.32 (+2) | 98 | N132*VSWATGR139 | iTRAQ8plex@N-term | 0.964 | 1.200 | 1.227 | 1.338 | 1.611 | 2.396 | 2.305 | 2.358 |
| User modH on Asn(N)@1 | ||||||||||||
| P07357|CO8A_HUMAN | 547.61 (+3) | 99 | G405GSSGWSGGLAQN417*R418 | iTRAQ8plex@N-term | 2.173 | 1.101 | 0.544 | 1.903 | 1.996 | 2.523 | 2.470 | 3.033 |
| User modH on Asn(N)@13 | ||||||||||||
| Q86VB7|C163A_HUMAN | 762.89 (+2) | 99 | A59PGWAN64*SSAGSGR71 | iTRAQ8plex@N-term | 1.503 | 1.276 | 0.861 | 0.722 | 1.903 | 3.862 | 2.412 | 4.575 |
| User modH on Asn(N)@6 | ||||||||||||
| P01033|TIMP1_HUMAN | 687.37 (+3) | 99 | F23VGTPEVN30*QTTLYQR37 | iTRAQ8plex@N-term | 0.601 | 1.492 | 1.316 | 1.614 | 1.626 | 2.695 | 2.238 | 9.126 |
| User modH on Asn(N)@8 | ||||||||||||
| P07996|TSP1_HUMAN | 558.64 (+3) | 99 | V1047VN1049*STTGPGEHLR1059 | iTRAQ8plex@N-term | 1.318 | 1.214 | 0.982 | 1.623 | 1.225 | 0.440 | 0.287 | 0.339 |
| User modH on Asn(N)@3 | ||||||||||||
| P07996|TSP1_HUMAN | 495.28 (+4) | 98 | V1047VN1049*STTGPGEHLR1059 | iTRAQ8plex@N-term | 1.088 | 1.517 | 0.904 | 0.748 | 1.113 | 0.260 | 0.449 | 0.226 |
| User modH on Asn(N)@3 | ||||||||||||
| iTRAQ8plex(T)@5 |
a The Confidence values in ProteinPilot software were shown. We set the peptide identification threshold as Conf. > 95.
b The identified N-glycosylation sites were indicated by asterisks and their amino acid numbers were also shown.
c Fold change values of the affinity to ConA lectin were calculated as relative differences from the average of three normal samples (NAvr). The ratio of more than 2-fold or less than 0.5-fold change was highlighted in red or blue, respectively.