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. 2010 May 7;116(7):1060–1069. doi: 10.1182/blood-2009-11-255075

Figure 3.

Figure 3

Id2 expression is deregulated in Gfi-1−/− mice. (A) CD4−CD8−, CD4+CD8+, and CD4+CD8− and CD4−CD8+ T lymphocytes were purified from Gfi-1+/+ and Gfi-1−/− thymuses by sorting. RNA was harvested from each population and analyzed for Id2 mRNA expression levels by real-time qRT-PCR. (B) B220+CD43− and B220+CD43+ B lymphocytes were purified from Gfi-1+/+ and Gfi-1−/− bone marrows by sorting. RNA was harvested from each population and analyzed for Id2 mRNA expression levels by real-time qRT-PCR. (C) LSKs, CMPs, GMPs, and MEPs were purified from Gfi-1+/+ and Gfi-1−/− mice by multicolor-based sorting. RNA was harvested from LSK, CMP, GMP, and MEP, and analyzed for Id2 mRNA expression levels by real-time qRT-PCR. All samples were normalized to GAPDH. *P < .05 in 2-sided t test. Three independent experiments were performed.