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. 2010 Jul 7;154(1):149–162. doi: 10.1104/pp.110.158865

Figure 2.

Figure 2.

Expression pattern of OsC6. A, Spatial and temporal expression analyses of OsC6 by qRT-PCR. Rice Actin1 (OsActin1) expression was used as a control. Total RNA was isolated from rice tissues: root, shoot, leaf, glume, lemma, palea, pistil at stage 13, and anthers at various stages (St 8, stage 8; St 9, stage 9; St 10, stage 10; St 11, stage 11; St 13, stage 13). B, GUS expression in anthers at stage 10 of wild-type (WT; left) and tdr (right) background. C, GUS expression was visible in immature anthers at different stages (from stage 8 to stage 13). D, GUS expression (arrowhead) in the tapetal cells at stage 10. The section was counterstained by Safranin O. E to H, In situ analyses of OsC6. E, A wild-type anther at stage 8 showing no OsC6 expression. F, A wild-type anther at stage 10 showing stronger OsC6 expression in tapetal cells. G, The expression of OsC6 decreased at stage 11. H, Successive section to that shown in F, probed with the OsC6 sense probe. A, Anther; E, epidermis; L, lemma; Msp, microspore; P, palea; T, tapetum; Tds, tetrads. Bars = 2 mm in B, 100 μm in C, and 20 μm in D to H.