Figure 7.
Transgenic expression of PTI-suppressing T3Es in Arabidopsis relieves PTI-induced injection restriction. Arabidopsis wild-type plants and Arabidopsis transgenic plants expressing the DC3000 T3Es HopAO1-HA or HopF2-HA were pretreated with 1 μm flg21 for indicated time periods and infiltrated with 3 × 108 cells/mL of P. fluorescens(pLN1965) expressing HopU1-CyaA. The higher amounts of cAMP in PTI-induced transgenic plants compared to the wild-type control indicated that these T3Es can relieve PTI-induced injection restriction. This experiment was repeated at least three times with similar results, and se bars are shown.