Table I. Relative expression levels of confirmed OCL1 target genes in OCL1-OE and OCL1-RNAi plants.
Oligo IDa and/or Gene Name | Trend in OCL1-OE (Plantlet) | Ratio OCL1-OE/Wild Typeb |
Ratio OCL1-RNAi/Wild Typec | Annotationf | Maize Gene Modelg | Class | ||
18-DAS Plantlet | Shoot Apexd | Immature Eard | 18-DAS Plantlete | |||||
OCL1 | Up | 23.80 | 4.00* | 3.46* | 0.51* | Transcription factor HD-ZIP IV family | GRMZM2G026643 | Transcription factor |
MZ00005958 | Down | 0.18 | 1.50 | 0.00* | 1.38* | Maize indole-3-glycerol phosphate lyase (Igl) | GRMZM2G015892 | Defense |
MZ00014373 | Down | 0.10 | 0.39* | 0.04* | 1.05 | MtN3/SALIVA-related transmembrane protein | GRMZM2G179349 | Envelope |
MZ00018561 | Down | 0.38 | 0.57* | 1.87 | 0.99 | Pro-rich protein; structural constituent of cell wall | GRMZM2G345700 | Envelope |
MZ00022171 | Up | 20.88 | 0.43 | 0.43 | 1.04 | Male sterility MS5 family protein; contains TPR domain | GRMZM2G075563 | Other |
MZ00024305 LtpII.12 | Up | 4.34 | 1.94* | 2.40* | 0.58* | Nonspecific lipid transfer protein (nsLTP) type 2 | GRMZM2G387360 | Lipid |
MZ00024414 | Down | 0.09 | 0.65* | 1.52 | 1.23* | MATE efflux family protein | GRMZM2G339488 | Envelope |
MZ00028617 | Down | 0.04 | 0.61* | 1.61 | 1.30* | Cytochrome P450; oxygen binding; CYP78A6-like | GRMZM2G034471 | Other |
MZ00029574 | Up | 7.35 | 1.25* | nd | 0.80* | Carboxylesterase; ATCXE18-like | GRMZM2G104141 | Lipid |
MZ00030315 | Up | 13.51 | 1.55* | 0.18 | 0.55* | Integral membrane family protein; contains DUF588 | GRMZM2G132128 | Envelope |
MZ00031783 ZmWBC11a | Up | 6.94 | 1.05 | 1.27* | 0.73* | ABC transporter; ABCG11/COF1/DSO/WBC11-like | GRMZM2G308860 | Lipid |
MZ00031955 | Up | 7.08 | 2.57* | 2.17* | 1.09 | SEC14/phosphoglyceride transfer family protein | GRMZM2G088501 | Lipid |
ZmFAR1 | Up | 2.78 | nd | nd | 1.21 | Fatty acyl-CoA reductase (alcohol-forming)/oxidoreductase; FAR1-like | GRMZM2G036217 | Lipid |
ZmWBC11b | Up | 2.69 | nd | nd | 1.00 | ABC transporter; ABCG11/COF1/DSO/WBC11-like | GRMZM2G096952 | Lipid |
ZmWBC11c | Up | 2.06 | nd | nd | 0.49* | ABC transporter; ABCG11/COF1/DSO/WBC11-like | GRMZM2G143668 | Lipid |
Identification number of the corresponding oligonucleotide deposited on the microarray.
Mean of a biological triplicate and a technical replicate; the expression values are reported relative to one of the wild-type samples.
Mean of a technical triplicate; the expression values are reported relative to the wild-type samples.
Asterisks indicate a trend of differential expression similar to the one observed in OCL1-OE plantlets. nd, Not determined.
Asterisks indicate a trend of differential expression opposite to the one observed in OCL1-OE plantlets.
Manually improved annotations from SwissProt, GenBank, Trembl, and InterPro databases.
Maize genome release 4a.53 of March 8, 2010 (http://www.maizesequence.org).