Table I. Growth rate (biomass accumulation), Glc consumption rate, and biomass composition of Arabidopsis cell suspension cultures grown under control conditions (21°C, no mannitol), at high temperature (29°C), and under hyperosmotic stress (171 mm mannitol).
All values are means ± sd from four replicates. Values indicated in boldface are significantly different (Student’s t test, P < 0.05) from the corresponding value for control cells.
Parameter | Control | 29°C | Hyperosmotic |
Biomass (g dry wt d−1 L−1 culture) | 2.3 ± 0.1 | 0.8 ± 0.1 | 0.9 ± 0.2 |
Glc consumption (g d−1 L−1 culture) | 4.1 ± 0.2 | 3.3 ± 0.1 | 1.6 ± 0.5 |
Biomass composition (%) | |||
Cell wall | 43 ± 5 | 35 ± 2 | 55 ± 2 |
Protein | 20 ± 2 | 16 ± 3 | 28 ± 4 |
Lipid | 11 ± 2 | 10 ± 1 | 11 ± 2 |
Starch | 5 ± 0.4 | 3 ± 0.4 | 5 ± 0.7 |
Soluble metabolites | 14 ± 1 | 21 ± 1 | 7 ± 1 |