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. 2010 Jul 12;154(1):197–210. doi: 10.1104/pp.110.159103

Figure 1.

Figure 1.

Functional complementation of fet3fet4 yeast. DEY1453-derived yeast strains transformed with pGEV-TRP and constructs containing AtYSL1, AtYSL3, OsYSL15, or ZmYS1 cDNA, or the empty pYES6/CT vector, were grown on synthetic defined medium containing variable conditions for Fe [Fe(II) or Fe(III)], chelator (DMA or NA), and β-estradiol (BE). Pairs of spots correspond to 10-fold and 100-fold dilutions of the original cultures. A, Results using Fe(II) and NA. Each plate contained the constituents indicated at the following concentrations: 50 μm Fe citrate, 3 μm Fe(II)SO4, 8 μm NA, 10 nm BE. B, Results using Fe(III) and PS. Each plate contained the constituents indicated at the following concentrations: 50 μm Fe citrate, 10 μm Fe(III)Cl3, 10 μm DMA, 40 nm β-estradiol. In addition, the Fe(II) chelator BPDS was added to some plates, as indicated. C, Results using Fe(III) and NA. Each plate contained the constituents indicated at the following concentrations: 50 μm Fe citrate, 10 μm Fe(III)Cl3, 15 μm NA, 40 nm BE.