Figure 6.
Enhanced susceptibility to Xav race 3 in CaMsrB2-silenced pepper plant. A, Bacterial growth in the silenced plants. Leaves of GFP (control), CaMsrB2-N-, or CaMsrB2-C-silenced plants were infiltrated with Xav race 3 (approximately 6 × 104 cfu mL−1), and the numbers of bacteria were counted for 6 DAI. Each bar shows the average and sd derived from two leaf discs of three independently silenced plants. Different letters indicate significant difference at 95% by Duncan’s multiple range test. B, RT-PCR analysis of defense-related genes (CaPR4 and CaPR10) and an antioxidant gene (CaSOD) in CaMsrB2-silenced pepper plants infiltrated with Xav race 3. CaActin was used to confirm equal total RNA amounts among samples.