Pharmacological inactivation of A2B receptors enhances inflammatory mediator production in polymicrobial sepsis. Blood and peritoneal lavage fluid was obtained from mice injected with the A2B receptor-specific antagonist MRS1754 or vehicle (DMSO) 16 h after the CLP procedure, and TNF-α (A), IL-1β (B), IL-12p40 (C), IL-6 (D), IL-10 (E), MIP-2 (F), and MCP-1 (G) concentrations were determined using ELISA (n = 10/group). All results (mean ± SEM) shown are representative of three experiments. *p < 0.05 versus vehicle-injected mice; **p < 0.01 versus vehicle-injected mice.