Skip to main content
. 2010 Apr 27;38(16):5366–5383. doi: 10.1093/nar/gkq285

Figure 8.

Figure 8.

Interaction between Prkacb and miR-372. (A) Prkacb 3′UTR contains a miR-372 target site, as shown in the left panel, and confirmed by Luciferase assays (right panel). pSi-Prkacb contains Prkacb 3′UTR. Experimental procedure is similar with that described in Figure 7C. (B) Immunoblotting assay indicated that over-expression of miR-372 (50 nM) results in down-regulation of PRKACB, thus reduces phosphorylation of CREB in Hep3B cells. (C) Differences in PRKACB expression level between Hep3B and HL-7702 cell lines were measured by immunoblotting assay. (D) PRKACB expression level was detected by immunoblotting assay after transfection with pcDNA3.1(+) vector as indicated in HL-7702 cells. (E) Prkacb or negative control siRNAs were co-transfected with Luc-HCP into Hep3B and HL-7702 cells. Luciferase assays were performed 24 h after transfection. Firefly luciferase activities were normalized to Renilla luciferase activities. (F) Endogenous HULC mRNA was quantified by real-time PCR and normalized to β-actin RNA. Results of RNAi are shown as fold change to the control. *P < 0.01 versus corresponding control cells. All results are shown as means of three independent experiments ± SD.