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. 2010 May 5;38(16):5527–5534. doi: 10.1093/nar/gkq327

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — MspJI digestion on genomic DNAs. M, pBR322 MspI digest; lane 1, Hela genomic DNA; lane 2, Hela gDNA+MspJI; lane 3, Hela gDNA+MspJI+activator;4, Arabidopsis gDNA; lane 5, Arabidopsis gDNA+MspJI; lane 6, Arabidopsis gDNA+MspJI+activator; lane 7, yeast gDNA; lane 8, yeast gDNA+MspJI; lane 9, yeast gDNA+MspJI+activator. All digestion reactions contain 1.5 µg of genomic DNA, 1.5 µg of MspJI in 30 µl volume. When indicated, a final concentration of 0.8 µM of DNA activator was supplemented to stimulate MspJI digestion. All reactions were incubated overnight at 37°C.