Table 2.
PEP results on modern, UV-irradiated and ancient DNA
| Samplea | Sequence identity | Average fragment size (bp)b | Extension products sequenced |
Proportion of molecules with blocking lesions (%) / per-base frequency of blocking lesions (%) | ||
|---|---|---|---|---|---|---|
| All | Terminated | Term (%) | ||||
| Modern horse, fraction 1 | All sequences | 91.6 | 6419 | 1028 | 16.0 | |
| Horse (87.9%) | 91.5 | 5643 | 627 | 11.1 | 0/0 | |
| Modern horse, fraction 2 | All sequences | 124.6 | 8885 | 1228 | 13.8 | |
| Horse (94.1%) | 124.4 | 8360 | 980 | 11.7 | 0/0 | |
| Modern horse, fraction 1, UV | All sequences | 87.6 | 4526 | 1927 | 42.6 | |
| Horse (94.1%) | 88.9 | 3440 | 1091 | 31.7 | 26/0.3 | |
| Ancient horse 1 >47k YBP | All sequences | 154.9 | 2023 | 1151 | 56.9 | |
| Horse (3.8%) | 153.8 | 77 | 32 | 41.6 | 39/0.3 | |
| Ancient horse 2 27k YBP | All sequences | 120.7 | 9030 | 1982 | 21.9 | |
| Horse (91.6%) | 120.8 | 8314 | 1608 | 19.3 | 10/0.1 | |
| Mammoth 43k YBP | All sequences | 154.6 | 8458 | 2762 | 32.7 | |
| Elephant (2.1%) | 115.4 | 174 | 22 | 12.6 | 2/∼0.0 | |
| Cave bear 42k YBP | All sequences | 113.3 | 1542 | 822 | 53.3 | |
| Dog (1.2%) | 125.0 | 18 | 7 | 38.9 | 36/0.4 | |
aAge of ancient samples is denoted in ‘years before present’ (YBP) as determined by C14 dating.
bThe average fragment size was inferred from complete extension products only.