Figure 4.

Repair joints formed in extracts depleted of pol λ and supplemented with pol λ DL. (A) Initial substrate. (B) Joints containing deletions (top strand) that were recovered in multiple clones. Bottom strand shows the initial overhangs. Bold letters indicate inferred gap filling. Dashes indicate bases that are present in the initial overhangs but missing in the repair product. Underlines indicate a 4-base microhomology. (C) Joints involving apparent ligation of heteroduplexes. At left, bottom strand shows overhangs of the initial substrate. Above it are two sequences that were recovered in a single clone, distinguishable by their abundance. Lower-case letters indicate apparent misinsertions. Diamond indicates apparent gap filling across the discontinuity in the template. Inferred ligated heteroduplexes are shown at right. (D) Additional sequences, each recovered in a single clone, including two with apparent untemplated insertions (overlines).