Table 1.

Steady-state analysis of single nucleotide incorporation by pol λ DL

Enzyme	Km (µM)	kcat (1/s)	kcat/Km (/s/µM)
Incorporation of dATP
Pol λ wt	0.39 ± 0.10	0.012 ± 0.004	3.0 × 10−2 ± 1.3 × 10−2
Pol λ DL	0.62 ± 0.126	0.0065 ± 0.001	1.0 × 10−2 ± 0.3 × 10−2
Incorporation of dGTP
Pol λ wt	7.4 ± 0.92	0.00014 ± 5.8 × 10−6	1.9 × 10−5 ± 2.5 × 10−6
Pol λ DL	28 ± 4.30	0.0064 ± 7.6 × 10−4	2.3 × 10−4 ± 0.4 × 10−4

The values for the kinetic constants are an average of 3–4 independent determinations.