Table 2.
Pol λ DL | Pol λ wt | |
---|---|---|
Mutant frequency × 10−2 | 54a | 21b |
Mutants sequenced | 50 | 103 |
Nucleotides sequenced | 20 350 | 41 921 |
Total changes | 618 | 253 |
Classes | Error rate × 10−4 | |
---|---|---|
Base substitutions | 120 (252) | 9 (38) |
1-nt deletions | 120 (239) | 45 (190) |
2-nt deletions | 5.9 (12) | 2.1 (9) |
1-nt additions | 3.4 (7) | 1.6 (7) |
Other changesc | (108) | (9) |
The number of occurrences is given in parenthesis.
aThe mutant frequency of pol λ DL represents one of seven independent determinations. The other six mutant frequencies were 43 × 10−2, 48 × 10−2 and 37 × 10−2 (reactions with 150 nM pol λ DL at pH 7.5), 39 × 10−2 and 50 × 10−2 (reactions with 50 nM pol λ DL at pH 8.5), 54 × 10−2 (reaction with 150 nM pol λ DL at pH 8.5).
bThe stated mutant frequency of pol λ wt, determined at the same time as the mutant frequency of pol λ DL, is identical to that reported earlier (39). The specificity of pol λ wt is from (39).
cClassified as ‘other changes’ are large deletions and complex errors involving substitution-deletion and substitution-addition errors.