Table 3.

Single nucleotide deletion error rates and the run length

Runs	Length (nucleotide)	Pol λ DLa		Pol λ wtb Error rate × 10−4
		Observed	Error rate × 10−4
One	206	89	86	13
Two	114	91	160	77
Three	57	40	140	82
Four/Five	30	19	127	81

^aThe error rates were calculated by dividing the number of observed deletions in the given category by the total number of template nucleotides present in runs of the listed lengths, among the 50 pol λ DL mutants sequenced [e.g. 89/(206 × 50) = 1-nt deletion rate at non-run sequences].

^bRates for pol λ wt are from (39).