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. 2010 Apr 28;38(16):5384–5395. doi: 10.1093/nar/gkq307

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Western blot experiments, recombinant expression of NANOG1 and NANOG2 variants in 293T cells and immunohistological experiments. (A) Western blot experiments using an antiserum against the C-terminal portion of NANOG protein revealed the expression of NANOG1 and NANOG2 protein variants in NTERA-2 cells, and NANOG2 in SEM cells. (B) Cloned Strep-affinity-tagged splice variants of NANOG1 and NANOG2. Predicted molecular weight of all expression constructs is indicated. Recombinant expression of NANOG1 splice variants A and B_b and NANOG2 splice variants D2_c and E in 293T cells were shown. Sizes of protein markers are indicated. (C) Immunohistological analysis of all recombinant NANOG1/2 protein variants expressed in Hela cells. Selected pictures from transfected cells were taken (20×). From left to right: phase contrast, anti-C NANOG antiserum, DAPI counter-staining and a merged picture is shown. All NANOG1/2 protein variants were localized within the nucleus of transfected cells.