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. 2010 May 5;38(16):5472–5478. doi: 10.1093/nar/gkq337

Figure 1.

Figure 1.

HOXA9 up-regulates miR-155 and BIC mRNA. (A) Western blot showing HOXA9 or HOXA9-NS protein expressed from a GFP-expressing retroviral vector after infection of Hoxa9−/−BM cells. (B) Northern gel analysis showing that miR-155 was ∼2.7-fold higher in wild-type BM cells compared to Hoxa9-deficient cells. (C) Retroviral-mediated expression of HOXA9 caused a 2.3-fold increase in miR-155, while a DNA binding mutant (HOXA9NS) did not significantly change miR-155 levels. The arrowhead indicates the miR-155 primary hairpin form. (D) The primary hairpin miR-155 is generated from the BIC transcript (34). Forced expression of HOXA9 produced a 10-fold increase in BIC message, while the HOXA9NS mutant did not significantly increase BIC mRNA.