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dDis3 cleavage of 3′-end-labeled RNAs confirms endoribonuclease activity. MBP-dDis31–394 (A) and MBP-dDis31–982 (B) cleave 3′-end-labeled polyA, polyC, polyU and polyN RNA substrates. Circular RNAs were a by-product of the 3′-end-labeling procedure. Note that reaction products for both proteins are RNA fragments. Data shown is representative of at least two independent experiments.
