Table 1.

Quantification of defects in HeLa cells following RNAi to Larp1, control dsRNA and transfection reagent alone

	Transfection reagent only control	Control (non-sense RNAi)	Larp1 RNAi
Apoptosis (preG1)	7.02% ± 0.84	7.8% ± 1.04	14.38% ± 1.93
Mitotic index	100% ± 0	100.8% ± 4.7	174.92% ± 20.18
Cell density	100% ± 0	75.8% ± 9.04	41.99% ± 4.35
Chromosome defects	2.14% ± 1.08	1.83% ± 0.67	6.33% ± 1.44

Following Larp1 RNAi, there is a greater percentage of sub G1 cells (indicative of apoptosis) and a higher number of cells with chromosome defects compared to controls. The mitotic index was also elevated following Larp1 RNAi.