| Target | Primary antibody | Secondary Antibody |
|---|---|---|
| Larp1 | Larp1 (Strategic Diagnostic) 1: 100 | Anti-Rabbit Cy5, (Invitrogen) 1: 500 |
| Microtubules | YL1/2 αTubulin antibody (Oxford Biotechnologies) 1: 50 | Anti-rat Alexa 488, (Molecular Probes) 1: 400 |
| Centrosomes | GTU88 γ-Tubulin (Sigma) 1: 200 | Anti-mouse Rhodamine, (JIR) 1: 100 |
| Cyclin B | Cyclin B (Sc-752 Santa Cruz) 1: 100 | Anti-rabbit Alexa 594, (Molecular Probes) 1: 500 |
| Total actin | Alexa Fluor Phalloidin (A22283-Invitrogen) 1: 500 | – |
| β-actin | β-actin (Ab8227-AbCam) 1: 100 | Anti-rabbit Alexa 594, (Molecular Probes) 1: 500 |
| γ-actin | γ-actin (A8481- Sigma) 1: 100 | Anti-mouse Alexa 488 (Molecular Probes) 1: 500 |
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