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. 2010 Jun 29;38(16):e163. doi: 10.1093/nar/gkq581

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Comparison of reverse transcriptase product lengths. Copy number of one sequence near the polyA (1348–1412) and one near the 5′-UTR (22–80), respectively, were determined by qPCR. Full-length cDNAs act as templates for both qPCRs, while incomplete cDNAs only template replication from the PCR primer pair located near the polyA tail. The ratio of the two transcript represents the proportions of cDNA molecules that reached full-length (superscript III: 76%, superscript II: 70%, omniscript: 65%).