Table 3.
Characteristics of the four classes of ex-siRNA
| sRNA class | Strand bias | Average log2 fold change from WT |
No. of exons | ex-siRNA (%) | 5′ Ua (%) | 3′ penult. Ub (%) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| dcl1− | dcl2− | Dcl1−/2− | rdrp1− | rdrp2− | ||||||
| Class 1 | −0.78 | 0.34 | −12.84 | −8.86 | 3.74 | −1.33 | 9 | 13.42 | 92.18 | 6.11 |
| Class 2 | −0.34 | 0.55 | −3.79 | −8.72 | −5.02 | 1.36 | 222 | 58.74 | 92.12 | 16.63 |
| Class 3 | 0.90 | −0.47 | 0.12 | −3.21 | −4.52 | −3.50 | 88 | 27.36 | 8.39 | 49.57 |
| Class 4 | 0.83 | −2.45 | −0.26 | −1.02 | −2.61 | −2.42 | 5 | 0.48 | 28.28 | 66.61 |
Different characteristics of ex-siRNAs from each classes are shown in the table. Strand bias indicates orientation to mRNAs, where 1 corresponds to all sRNAs in the same orientation as the mRNA, 0 to equal mixture of sRNAs on both strands and −1 to all sRNAs antisense to mRNAs. Numbers in bold indicate a higher than 4-fold down-regulation in the corresponding mutants relative to wild type.
aThe percentage of redundant reads that contain a uracil in the 5′ most position.
bThe last column shows the percentage of redundant reads that contain a uracil in the 3′ penultimate position.