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. 2010 Apr 28;38(16):5535–5541. doi: 10.1093/nar/gkq301

Table 3.

Characteristics of the four classes of ex-siRNA

sRNA class Strand bias Average log2 fold change from WT
No. of exons ex-siRNA (%) 5′ Ua (%) 3′ penult. Ub (%)
dcl1 dcl2 Dcl1/2 rdrp1 rdrp2
Class 1 −0.78 0.34 −12.84 −8.86 3.74 −1.33 9 13.42 92.18 6.11
Class 2 −0.34 0.55 −3.79 −8.72 −5.02 1.36 222 58.74 92.12 16.63
Class 3 0.90 −0.47 0.12 −3.21 −4.52 −3.50 88 27.36 8.39 49.57
Class 4 0.83 −2.45 −0.26 −1.02 −2.61 −2.42 5 0.48 28.28 66.61

Different characteristics of ex-siRNAs from each classes are shown in the table. Strand bias indicates orientation to mRNAs, where 1 corresponds to all sRNAs in the same orientation as the mRNA, 0 to equal mixture of sRNAs on both strands and −1 to all sRNAs antisense to mRNAs. Numbers in bold indicate a higher than 4-fold down-regulation in the corresponding mutants relative to wild type.

aThe percentage of redundant reads that contain a uracil in the 5′ most position.

bThe last column shows the percentage of redundant reads that contain a uracil in the 3′ penultimate position.