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. 2010 Apr 29;38(16):5554–5568. doi: 10.1093/nar/gkq305

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Submitochondrial localization of exogenous ERAL1-HA. Mitochondria were incubated in hypotonic buffer for disruption of the outer membranes without (lanes 3 and 4) or with a non-ionic detergent, Triton X-100 (lane 5). Then the mitochondria were digested with proteinase K (lanes 2, 4 and 5). The indicated proteins were detected by immunoblotting. EndoG, BAP37 and TFAM are markers for the mitochondrial IMS, IM and matrix, respectively.