Table 1.
List of proteins identified in complex with ERAL1
| Swiss-Prot accession number | Gene symbol | Name | No. of peptides matched | % Sequence coverage | MW | PI | Nucleoid type |
|---|---|---|---|---|---|---|---|
| P38646 | HSPA9 | Stress-70 protein | 8 | 17 | 73 920 | 5.87 | Class I |
| Q15031 | LARS2 | Leucyl-tRNA synthetase | 4 | 4 | 1 02 823 | 8.46 | |
| Q9BW92 | TARS2 | Threonyl-tRNA synthetase | 1 | 1 | 81 841 | 6.9 | |
| P31327 | CPS1 | Carbamoyl-phosphate synthase | 8 | 4 | 1 65 975 | 6.30 | Class I |
| P42704 | LRPPRC | Leucine-rich PPR motif-containing protein | 7 | 5 | 1 59 003 | 5.81 | Class I |
| Q00059 | TFAM | Transcription factor A | 1 | 4 | 29 306 | 9.74 | Class I |
| P34897 | SHMT2 | Serine hydroxymethyltransferase | 3 | 5 | 56 414 | 8.76 | Class I |
| P49411 | TUFM | Elongation factor Tu | 3 | 8 | 49 852 | 7.26 | Class I |
| P10809 | HSPD1 | 60 kDa heat shock protein | 24 | 37 | 61 187 | 5.70 | Class II |
| Q99623 | PHB2 | Prohibitin-2 | 7 | 24 | 33 276 | 9.83 | Class II |
| P40926 | MDH2 | Malate dehydrogenase | 2 | 7 | 35 965 | 8.92 | Class III |
| P54886 | ALDH18A1 | Delta-1-pyrroline-5-carboxylate synthetase | 3 | 3 | 87 989 | 6.66 | |
| Q12931 | TRAP1 | Heat shock protein 75 kDa | 3 | 4 | 80 345 | 8.30 | |
| P31040 | SDHA | Succinate dehydrogenase [ubiquinone] flavoprotein subunit | 1 | 2 | 73 672 | 7.06 | |
| Q5R1W8 | VIM | Vimentin | 6 | 17 | 53 677 | 5.03 | |
| P00367 | GLUD1 | Glutamate dehydrogenase 1 | 6 | 12 | 61 701 | 7.66 | |
| P55084 | HADHB | Trifunctional enzyme subunit γ | 1 | 2 | 51 547 | 9.45 | |
| O75616 | ERAL1 | GTP-binding protein era homologue | 20 | 32 | 48 833 | 9.05 | |
| P60709 | ACTB | Actin, cytoplasmic 1 | 5 | 15 | 42 052 | 5.29 | |
| P82650 | MRPS22 | 28S ribosomal protein S22 | 1 | 3 | 41 425 | 7.70 | |
| P04406 | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | 3 | 12 | 36 201 | 8.57 | |
| P48047 | ATP5O | ATP synthase subunit O | 4 | 24 | 23 377 | 9.97 | |
| Q9UIK4 | DAPK2 | Death-associated protein kinase 2 | 2 | 4 | 43 042 | 6.45 | |
| Q92665 | MRPS31 | 28S ribosomal protein S31 | 2 | 6 | 45 405 | 9.32 | |
| Q46199 | MTIF2 | Translation initiation factor IF-2 | 3 | 2 | 81 837 | 6.71 | |
| P82909 | MRPS36 | 28S ribosomal protein S36 | 1 | 4 | 11 459 | 9.99 |
The columns correspond to their accession number, gene symbol, name, number of unique peptide matched, percent sequence coverage, estimated MW and PI identified by LC-MS/MS analysis. We observed top three proteins in non-crosslinkng condition. Putative nucleoid proteins as recently classified by Bogenhagen et al. (29). Class I: core nucleoid protein, Class II: proteins seen in native but not cross-linked nucleoids and Class III: proteins seen in cross-linked but not native nucleoids.