Table 5.
Analysis of RNase T1 quantitative signature digestion products from E. coli
| tRNA | qSDP sequence | Experimental I18/I16 | CV (%) |
|---|---|---|---|
| Decrease in relative abundance | |||
| Cys | CA[ms2i6A]AΨCCGp | 0.75 | 19 |
| Cys | U[s4U]AACAAAGp | 0.67 | 25 |
| Tyr 1, 2 | ACUQUA[ms2i6A]AΨCUGp | 0.60 | 18 |
| Increase in relative abundance | |||
| Gly 1 | AUUCCCUUCGp | 1.44 | 26 |
| Gly 2 | CCU[Um]CCAAGp | 1.28 | 24 |
| Gly 3 | AAUAGp | 1.88 | 13 |
| Ser 1, 4, 5 | AAAGp | 2.43 | 18 |
| Ser 1, 2 | A[ms2i6A]AACCGp | 1.42 | 10 |
| No change in relative abundance | |||
| Ala 1, 2 | [m7G]UCUGp | 1.17 | 15 |
| Arg 1, 2 | [m2A]ACCGp | 1.04 | 20 |
| Asn | UCCUCUGp | 1.21 | 15 |
| Glu 1, 2, 3 | AAUCCCCUAGp | 1.06 | 15 |
| Glu 1, 2, 3 | UCCCCUUCGp | 1.20 | 15 |
| Leu 1 | UCCCCCCCCUCGp | 1.09 | 15 |
| Phe | AA[ms2i6A]AΨCCCCGp | 1.31 | 13 |
| Phe | A[s4U]AGp-3′ | 1.22 | 25 |
| Phe | U[m7G][acp3U]CCUUGp | 1.21 | 25 |
| Ser 3 | CUCCC[s2C]UGp | 1.00 | 17 |
| Trp | UCUCUCCGp | 1.21 | 27 |
| Trp | U[Cm]UCCA[ms2i6A]AACCGp | 1.29 | 22 |
| Val 1 | AU[s4U]AGp | 0.86 | 16 |
| Indeterminate | |||
| His | UU[m7G]UCGp | 1.80 | 24 |
| His | AAUCCCAUUAGp | 1.36 | 21 |
| His | [m2A]ΨΨCCAGp | 1.00 | 27 |
| Ini 1, 2 | TΨCAAAUCCGp | 1.51 | 23 |
| Ini 1, 2 | [Cm]UCAUAACCCGp | 0.97 | 41 |
CV, coefficients of variation.