Table 1.

Early definitive hematopoietic progenitor cell cycle

CD41+/CD45−c-kit+	Day 6			Day 7			Day 8
	G1	S	G2	G1	S	G2*	G1	S†	G2
Wt	82.9 ± 7.64	14.34 ± 7.44	2.24 ± 3.16	92.45 ± 10.68	9.76 ± 3.88	0.04 ± 0.06	79.87 ± 6.9	25.27 ± 3.58	1.72 ± 2.32
Hhex−/−	83.2 ± 5.09	8.71 ± 5.5	10.74 ± 7.3	93.65 ± 6. 57	5.81 ± 7.91	5.14 ± 0.41	79.2 ± 6.16	11.12 ± 5.44	11.51 ± 7.95

Cell-cycle profiles for early CD41⁺CD45⁻c-kit⁺ progenitors on days 6 to 8. Each cell within the table represents the average percentage within each phase of cell cycle ± the standard deviation, as determined from 3 experiments. Gating for cell cycle is shown in supplemental Figure 4. Hhex^−/− CD41⁺CD45⁻c-kit⁺ cells are accumulating in the G₂ phase of the cell cycle. Day 7 shows a significant difference in G₂ phase of the cell cycle, with Hhex^−/− CD41⁺CD45⁻c-kit⁺ cells accumulating in G₂ of the cell cycle. In addition, day 8 cocultures show that wt CD41⁺CD45⁻c-kit⁺ have a significantly higher number of cells in S phase, but Hhex^−/− CD41⁺CD45⁻c-kit⁺ on average still have higher number of cells in G₂.

^*P < .005, 2-tailed t test.

^†P < .05, 2-tailed t test.