Figure 5.

Prevention and restoration of oral tolerance by blockade of B7-H1/CD80 interaction. B6 mice were given drinking water supplemented with OVA protein (○ or ●) or without OVA (□) from day 0 to day 7. On day 14, the mice were immunized subcutaneously with OVA protein emulsified in CFA. The mice were also treated intraperitoneally with 150 μg of 43H12 (●) or control rat IgG (○) on days 0, 4, 8, and 12 (A) or on days 14 and 17 (B). On day 21, draining LN cells were harvested from the mice and cultured with the indicated doses of OVA protein. After 48 hours, production of IFN-γ, IL-2, and IL-4 in culture supernatant was measured by ELISA. IL-17 level was measured 24, 48, and 72 hours after culture with 25 μg/mL OVA protein. Proliferative activity was assessed by an incorporation of ³H-thymidine. All experiments were repeated at least 3 times. Representative data are shown as mean ± SD of triplicate wells in each group.