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. 2010 Jul 27;103(5):715–726. doi: 10.1038/sj.bjc.6605823

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Copyright © 2010 Cancer Research UK

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Inhibition of endogenous expression of ANO1 decreases cell migration. (A) Transfection of siRNAs 2, 5 and 7 inhibits ANO1 protein expression in SCC-25 cell line. Cells transfected with siRNAs for 48 h were scrape-wounded. Proteins were extracted 1 and 24 h after cell injury and were analysed by western blotting. (B) ANO1 inhibition decreases migration of SCC-25 cells – shown by in vitro wound healing on SCC-25 transfected with siRNAs (N=2). ^*Indicates statistical significance (P-value <0.05).