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. 2010 Sep 13;5(9):e12706. doi: 10.1371/journal.pone.0012706

Table 1. Interactions between MPF, MAPK and RhoA on enrichment of actin and myosin during ooplasmic protrusion following different treatments of goat oocytes.

Treatments Protrusion MPF RhoA MAPK Actin enrichment Myosin enrichment Spindle integrity
D + + + + + +
D− +
D+U + +
D+Y +
D+R
M + + NA + + + +
M+U + NA +
M+Y + +
M+Y+OA + + + +
M+R NA +
OA.5h + + +
OA4h + + + + + + +
OA4h+R + + +
OA4h+U NA +

Abbreviations: D: 0.8 ng/ml demecolcine; D-: Oocytes without protrusion after D; U: 20-µM U0126; Y: 400-µM Y27632; R: 400-µM ROS; M: 5-µM MG132; C: 1-mM caffeine; OA.5h: 0.5-µM OA for 0.5 h; OA4h: 0.1-µM OA for 4 h; +: In the presence of; NA: Not assayed. All treatments were performed for 30 min unless otherwise specified.