Figure 2. Hydrogen peroxide production in liver mitochondria from control (black bars, n = 7) and fat-1 (grey bars, n = 5) mice.

All measurements were completed on freshly isolated mitochondria. H₂O₂ production was monitored in mitochondria respiring on succinate (A), succinate/glutamate/malate (B), glutamate/malate (C), or pyruvate/malate (D). S, succinate; S/G/M, succinate/glutamate/malate; G/m, glutamate/malate; P/M, pyruvate malate; Rot, rotenone; AA, antimycin a. *, †, ‡, § indicates significant differences (P<0.05) between control and fat-1 mitochondria respiring on succinate, succinate/glutamate/malate, glutamate/malate and pyruvate/malate, respectively. Data are presented as means ± SEM.