Figure 1. Behavioural responses to acute mechanical or noxious thermal stimuli in naïve animals.

Adult male and female Mc1r^e and their littermate controls Tg-Mc1r^e, or A^y-Jkn1 and their littermate control non-A^y-Jkn1 mice were assessed for responses to acute noxious thermal or non-noxious mechanical stimuli. The paw withdrawal latency (PWL; n = 8–11 per genotype) [A], paw withdrawal threshold (PWT; n = 8–11 per genotype) [B] as measures of thermal hyperalgesia and mechanical allodynia respectively of both hind-paws (right and left hind-paw responses combined) in naïve animals are shown as mean responses ± SEM. Significant differences between Mc1r^e and A^y-Jkn1 and their respective controls were only seen in female Mc1r^e mice in [A] paw withdrawal latency (***P<0.01).