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. 2010 May 27;21(7):891–901. doi: 10.1089/hum.2009.148

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Copyright 2010, Mary Ann Liebert, Inc.

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FIG. 1. — Capsid-modified Ad vectors. The constructs used in this study are replication-defective E1^–,E3^– serotype 5 Ad gene transfer vectors containing (5′ to 3′) the cytomegalovirus immediate early promoter/enhancer (CMV), a marker gene, and the simian virus 40 poly(A) stop signal. Top: Ad5 genome. Middle: AdYFP-pIX/V expresses YFP as a transgene and has the V antigen coding sequence fused in frame to the C terminus of protein IX. Bottom: AdLacZ-pIX/F1 expresses LacZ as a transgene and has the coding sequence for the F1 capsular antigen fused in frame to the C terminus of protein IX. Expression of the pIX/V or pIX/F fusion protein is under the control of the pIX promoter.